Page 33 - IJSA, Vol. 4, No 2, 2021

International Journal of Science Annals, Vol. 5, No. 1-2, 2022
рrint ISSN: 2617-2682; online ISSN: 2707-3637; DOI:10.26697/ijsa

SOCIAL AND BEHAVIORAL SCIENCES. Health Care Sciences

ORIGINAL RESEARCH
Blast Crisis in Chronic Myeloid Leukemia:

An Immunophenotypic Analysis

Authors’ Contribution: 1 ABCDEF 1 ABCDEF 1 BCDE
A – Study design; Singh Ak. , Singh An. , Kanaujia S. ,
B – Data collection; Kushwaha R. 1 ABCDEF , Singh U. S. 1 ADE
C – Statistical analysis;
D – Data interpretation; 1 King George’s Medical University, India
E – Manuscript preparation;
F – Literature search; Received: 20.09.2022; Accepted: 27.10.2022; Published: 25.12.2022
G – Funds collection
Abstract
Background and There are two different phases of untreated chronic myeloid leukemia – chronic
Aim of Study: phase, and blast crisis – according to the World Health Organization
classification of Hematolymphoid tumors. The blast cells in the chronic myeloid
leukemia blast crisis can express myeloid, lymphoid, bi-phenotypic, monocytic,
megakaryocytic, and erythroid phenotypes. The immunophenotype of blast
population determines how chronic myeloid leukemia – blast crisis patients
respond to treatment, hence flowcytometric examination is required.
The aim of the study: to assess immunophenotyping outcomes of flowcytometry
performed on the chronic myeloid leukemia – blast crisis.
Material and Methods: A five-year retrospective descriptive analysis was carried out in Pathology
Department at King George’s Medical University Lucknow, India (2017-2021).
The patient’s peripheral blood and bone marrow aspirate samples were analyzed.
Clinical, hematological, and immunophenotypic data were retrieved. The flow
cytometry samples were prepared using the standardized “lyse-stain-wash”
method.
Results: A total of 43 cases of chronic myeloid leukemia – blast crisis were retrieved from
the departmental archive in 5 years. The mean age of study population was
39.62±14.86 years. There were 24 males and 19 females. 27 patients were
diagnosed with myeloid blast crisis, 14 cases of B-lymphoid blast crisis and 2
cases of mixed phenotypic acute leukemia.
Conclusions: Identification of the blast lineage of patients with chronic myeloid leukemia – blast
crisis is crucial since the existence of atypical blast phenotypes influences the
disease treatment and prognosis.
Keywords: immunophenotyping, flowcytometry, chronic myeloid leukemia, lymphoid blast,
myeloid blast, mixed phenotypic acute leukemia
Copyright: © 2022 Singh Ak., Singh An., Kanaujia S., Kushwaha R., Singh U. S. Published
by Archives of International Journal of Science Annals
DOI and UDC DOI https://doi.org/10.26697/ijsa.2022.1-2.3 UDC 616-005.9
Conflict of interests: The authors declare that there is no conflict of interests
Peer review: Double-blind review
Source of support: This research did not receive any outside funding or support
Information about Singh Akanksha (Corresponding Author) – https://orcid.org/0000-0001-5398-
the authors: 6203; [email protected], MD, Senior Resident, Department of
Pathology, King George’s Medical University, Lucknow, India.
Singh Anurag – https://orcid.org/0000-0002-1979-8847; MD, Senior Resident,
Department of Pathology, King George’s Medical University, Lucknow, India.
Kanaujia Sweta – https://orcid.org/0000-0003-3369-1664; MD, Resident,
Department of Pathology, King George’s Medical University, Lucknow, India.
Kushwaha Rashmi – https://orcid.org/0000-0002-0581-2177; MD, Professor,
Department of Pathology, King George’s Medical University, Lucknow, India.
Singh Uma Shankar – https://orcid.org/0000-0002-9748-9484; MD, Professor,
Head of the Department of Pathology, King George’s Medical University,
Lucknow, India.

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